Facilities

The Exercise Science Research Center (ESRC) operates as the research facility and is an academic component of the Department of Health, Human Performance and Recreation. The ESRC serves as the responsible multi-disciplinary unit for the initiation and coordination of research, outreach education and service programs. This lab is one of the most comprehensive research facilities in the area.

The Human Performance Laboratory provides ~6,000 sq ft of research space that allows for high level health-related clinically-relevant research.  

Resources in the ESRC include (but are not limited to):
-       Doppler ultrasound imaging with vascular probes
-       Treadmills
-       Cycle ergometers, including electronically-braked ergometers (RacerMate, Inc.)
-       12-lead ECG (Quinton Q-Stress®) integrated with computer for electronic recording
-       Venous blood collection supplies including needles, catheters, tubes, etc.
-       Equipment to measure blood volume (co-oximetry analyzer for carbon monoxide measures, glass tube setup and carbon monoxide)
-       Equipment to measure hemodynamic variables including hydration status (hematocrit and hemoglobin for plasma volume changes, osmometer, electrolyte analyzer, lactate and glucose analyzer, refractometer, HbA1c etc.)
-       Non-invasive blood pressure (Tango from SunTech Medical) and heart rate (Polar, Inc.) monitoring
-       End-tidal CO2 monitoring system
-       Refrigerators and freezers (-20 and -80°C) to store samples (with back-up generators)
-       Platform and medical scales for mass measurements
-       Infusion pumps and supplies to perform mechanistic microdialysis studies
-       Skin blood flow measurement system (Moor Instruments)
-       Control of local skin temperature (Perimed)
-       Capacitance hygrometry sweat rate systems (Vaisala)
-       Whole-body heating/cooling systems for passive heat stress (tube-lined suits from Meg-Eng, Ottawa, Canada)
-       Skin (Omega Engineering, Inc) and internal (rectal and intestinal) temperature monitoring.
-       Dual-Energy X-Ray Absorptiometer (DEXA) machine. 
-       Metabolic cart (ParvoMedics)  capable of measuring oxygen uptake via open circuit spirometry and ventilation
-       Non-invasive cardiac output measures (CO2-rebreathing) integrated with the metabolic cart.

Within the laboratory there is an environmental chamber (~270 sq ft) which is capable of controlling temperature (4 to 44°C) and humidity (30 to 75%). The chamber is designed such that the cardiovascular and thermoregulatory equipment necessary for data collection can be moved and utilized in this space.

All signals are collected via an 16 channel data recording and analysis system (PowerLab and Chart) connected to a high-speed computer. To prevent the loss of data during a temporary power disruption, data collection computers are on a battery-backup system. Data collection computers have removable storage capabilities (CD-RW and external hard drive) for data backup.

As part of the ESRC we have a recently renovated wet lab (~1,000 sq ft) with the following resources & equipment:

  1. Nikon TiS Inverted Microscope System with X-Cite 120 LED Fluorescent System; LWD objectives of 4, 10, 40X; 100X oil immersion objective; fluorescent light cubes corresponding to DAPI, FITC, TRITC and Texas Red equivalent channels, NIS-Elements Basic Research Acquisition and analysis package
  2. ABI Step One RealTime PCR Thermocycler
  3. LiCor CDigit Western Blot Scanner
  4. 3x Apparatuses for electrophoresis of agarose gels
  5. 4x Life Technologies Bolt Polyacrylamide Gel Electrophoresis apparatuses
  6. 4x Power supplies for electrophoresis (BioRad)
  7. VWR microcentrifuge
  8. -80°C and -20°C freezers
  9. 4ºC refrigerators
  10. Harvard Apparatus Force Transducer
  11. Schuler Tissue Bath System
  12. Symphony pH meters
  13. 3x hotplates/stirrers
  14. Beckman Coulter XL-22R centrifuge
  15. Incubators and water baths
  16. Tissue Tek homogenizers
  17. Applied Biosystems PCR thermocycler
  18. Techne Genius PCR thermocycler
  19. Qubit flurometer
  20. 2x circulating water baths
  21. BioTek microplate reader with Take3 microvolume microplate for nucleic acid quantification
  22. DEXA machine with small animal software 
  23. 2x Full set of manual and automatic micropipettes
  24. nutating mixer, digital dry baths
  25. Napco autoclave
  26. FL800X microplate fluorescence reader
  27. Olympus CKX41 inverted microscope with digital color camera tied to Nikon BR software
  28. Purelab ultra water purification unit
  29. Explosion proof refrigerator/freezer 
  30. High speed centrifuge
  31. 2x analytical balance (Mettler Toledo, 0.1mg)
  32. 2x 5’ Chemical fume hood
  33. 2x water bath, 3x hot plates
  34. Grass S88 Stimulator
  35. Columbus Instruments 8 wheel home cage mouse running system with computer driven interface (maintained in CLAF A36).
  36. Research Animal Treadmill (maintained in CLAF A36).
  37. ThermoFisher 1300 Series A2 BSC Class II Biosafety Cabinet for Cell Culture
  38. ThermoFisher MaxQ 4450 Benchtop Orbital Shaker/Incubator
  39. ThermoFisher Forma Series II Water Jacketed CO2 Incubator with IR Sensor
  40. ThermoFisher Isotemp Dual Chamber Water Bath
  41. Sorvall ST8 Centrifuge

 In addition to these resources, PI's have access to the University's core facilities which includes an animal facility.